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Image Search Results
Journal: Cell
Article Title: RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, Using Annexin A11 as a Molecular Tether
doi: 10.1016/j.cell.2019.08.050
Figure Lengend Snippet:
Article Snippet: Cells were seeded and maintained in Cortical Neuron Culture Media, composed of
Techniques: Recombinant, Transgenic Assay, shRNA, Sequencing, Modification, Software
Journal: Molecular neurobiology
Article Title: Exosomes derived from mesenchymal stromal cells promote axonal growth of cortical neurons
doi: 10.1007/s12035-016-9851-0
Figure Lengend Snippet: A histogram (A) shows particle sizes of MSC-exosomes analyzed by the qNano system. Western blot analysis (B) shows the proteins of Alix and Hsp70 in MSC-exosomes (Exo) and supernatants (Sup). The schematic of a microfluidic culture device and a representative immunofluorescent image (C) show the pNFH+ cortical neurons and axons (green) in the cell body compartment (soma) and pNFH+ axons (green) in the axonal compartment (axon). The compartments are connected by 450 μm long microgrooves (C, microgrooves). DAPI labeled nuclei of cortical neurons (blue) were only present in the cell body compartment (C, soma). Panels D and E show representative images of pNFH+ axons in the axonal compartment (D, green) and quantitative data of the average axonal length (E) from the control (Ctl) and MSC-exosome (Exo) groups when the exosomes were applied into the soma compartment for 24 and 48h. * p<0.001 vs control. Panels F and G show representative time-lapse microscopic images of the growth cone extension (F) and quantitative data of growth cone elongation (G) in the axonal compartment during 60 min from the control (Ctl) and MSC-exosome (Exo) groups. Yellow and red arrows in panel F indicate starting (0 min) and ending (60 min) points, respectively. The representative time-lapse microscopic images (F) were acquired from 24h after soma application of the exosomes, whereas quantitative data (G) were acquired for 24 and 48h after exosome application. *p<0.05 vs control. Sample size in G represents the number of the growth cone from every independent experiment.
Article Snippet: The cortical neurons were then seeded in a
Techniques: Western Blot, Labeling, Control